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Xenbase Image ID: 150072

Fig. 2. Expression of prickle3 transcripts in Xenopus early embryos. Embryos were subjected to whole-mount in situ hybridization with Pk3 anti-sense RNA probe or sense probe as a control. Pk3 transcripts are present in the animal pole ectoderm of stage 10 gastrulae (A), and non-neural ectoderm of stage 15 neurulae (B). (C) Stage 18 embryo. Epidermal ectoderm is strongly positive (arrowhead), as compared to neuroectoderm. (D) Stage 21 embryo, anterior region, with staining in the profundal and posterior region placodes (arrowhead). (E) Stage 24 embryo, the otic vesicle and the posterior placodal region are positive (arrowheads). (F) Stage 26 embryos, tailbud. An embryo hybridized with the sense probe is shown on bottom. Anterior is to the left. Lateral view is shown in all panels, except dorsal view in C. (G, H) Cross-sections of stage 18 embryos. Arrowheads point to the staining in neural folds (G) and in both inner and outer layers of the epidermis (H). Positions of nuclei in the epidermis are outlined by dotted lines. Abbreviations: n, notochord, s, somites, np, neural plate.

Image published in: Ossipova O et al. (2015)

Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.

prickle3.Lpk3, prickle-3X. laevisThroughout NF stage 10animal pole
prickle3.Lpk3, prickle-3X. laevisThroughout NF stage 15epidermis
non-neural ectoderm
prickle3.Lpk3, prickle-3X. laevisThroughout NF stage 18epidermis
non-neural ectoderm
neural fold
prickle3.Lpk3, prickle-3X. laevisThroughout NF stage 21cranial placode
profundal placode
prickle3.Lpk3, prickle-3X. laevisThroughout NF stage 24cranial placode
posterior placodal area
otic placode
prickle3.Lpk3, prickle-3X. laevisSometime during NF stage 26 to NF stage 27epidermis
cranial placode
otic placode
cranial neural crest
pronephric mesenchyme
tail bud

Image source: Published

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