Larger Image Fig. 2. Expression of prickle3 transcripts in Xenopus early embryos. Embryos were subjected to whole-mount in situ hybridization with Pk3 anti-sense RNA probe or sense probe as a control. Pk3 transcripts are present in the animal pole ectoderm of stage 10 gastrulae (A), and non-neural ectoderm of stage 15 neurulae (B). (C) Stage 18 embryo. Epidermal ectoderm is strongly positive (arrowhead), as compared to neuroectoderm. (D) Stage 21 embryo, anterior region, with staining in the profundal and posterior region placodes (arrowhead). (E) Stage 24 embryo, the otic vesicle and the posterior placodal region are positive (arrowheads). (F) Stage 26 embryos, tailbud. An embryo hybridized with the sense probe is shown on bottom. Anterior is to the left. Lateral view is shown in all panels, except dorsal view in C. (G, H) Cross-sections of stage 18 embryos. Arrowheads point to the staining in neural folds (G) and in both inner and outer layers of the epidermis (H). Positions of nuclei in the epidermis are outlined by dotted lines. Abbreviations: n, notochord, s, somites, np, neural plate. Image published in: Ossipova O et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue prickle3.L pk3, prickle-3 X. laevis Throughout NF stage 10 animal pole prickle3.L pk3, prickle-3 X. laevis Throughout NF stage 15 epidermis non-neural ectoderm prickle3.L pk3, prickle-3 X. laevis Throughout NF stage 18 epidermis non-neural ectoderm neural fold prickle3.L pk3, prickle-3 X. laevis Throughout NF stage 21 cranial placode profundal placode prickle3.L pk3, prickle-3 X. laevis Throughout NF stage 24 cranial placode posterior placodal area otic placode prickle3.L pk3, prickle-3 X. laevis Sometime during NF stage 26 to NF stage 27 epidermis cranial placode otic placode cranial neural crest pronephric mesenchyme tail bud Image source: Published Permanent Image Page Printer Friendly View XB-IMG-150072