XB-PHENO-27144
Xla Wt + ctnnb1 MO, Azbazdar Y and De Robertis EM (2024)
| Experiment + Assay: |
Xla Wt + ctnnb1 MO +
NF11.5 (RT-PCR)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
ctnnb1 MO1
|
ctnnb1
|
NF stage 3 (4-cell)
|
left-ventral blastomere
|
|
morpholino injection
|
ctnnb1 MO1
|
ctnnb1
|
NF stage 3 (4-cell)
|
right-ventral blastomere
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
RT-PCR
|
NF stage 11.5
|
|
|
|
|
Expression Phenotypes (4):
|
|
absent
chrd.L
expression in dorsal marginal zone
|
|
decreased amount
sia1.L
expression in dorsal marginal zone
|
|
increased amount
szl.L
expression in ventral marginal zone
|
|
increased amount
ventx1.2.L
expression in ventro-lateral marginal zone
|
|
