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XB-PHENO-25851

Xtr.Tg(pbin7:GFP) + hif1a MO, Patel JH et al. (2022)

Experiment + Assay: Xtr.Tg(pbin7:GFP) + hif1a MO + NF18 (in situ hybridization)
Background: Xtr.Tg(pbin7:GFP)Wills
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection hif1a MO3 hif1a
 NF stage 2 (2-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 18  
Anatomical Phenotypes: abnormal neural plate morphology , abnormal Wnt signaling pathway
Expression Phenotypes (2):
mislocalised en2 expression in hindbrain
mislocalised otx2 expression in forebrain
Source: Fig. 5 H
Article: XB-ART-58820, PubMed