Xenbase Image ID: 82388

FIGURE 2. Xenopus CD38 is developmentally regulated. A, RT-PCR analysis of Xenopus CD38 expression in staged Xenopus embryos. Ubiquitously expressed ornithine decarboxylase (ODC) and RNA (stage 33) without reverse transcriptase (−RT) were used as controls. Embryos were collected as blastulae (stage 8), gastrulae (stages 12 and 13), neurulae (stages 15 and 22), and tailbud stages (stages 25 and 33). B, whole mount in situ hybridization for CD38 expression (purple stain) in Xenopus embryos (anterior to left and posterior to right). B, dorsal view of a stage 16 embryo. C, cross section of a stage 16 embryo with neural plate (np), notochord (n), somites (s), and endoderm (e) indicated. D, lateral view of a stage 23 embryo. E, lateral view of a stage 30 embryo. F, time courses for endogenous ADP-ribosyl cyclase activities in Xenopus homogenates from stage 11 and stage 36 embryos, showing production of ADPR from NAD, cGDPR from NGD, and NAADP from NADP and nicotinic acid (NA). Nic, nicotinamide. G, stage-dependent NADase activity (ADPR production from NAD) in Xenopus embryo homogenates. H, region-specific NADase activity in fragments from stage 36 embryos. H, head; D, dorsal abdomen; V, ventral abdomen; T, tail. Error bars represent mean S.E. Image published in: Churamani D et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue cd38.L X. laevis Throughout NF stage 16 mesoderm cd38.L X. laevis Throughout NF stage 23 mesoderm paraxial mesoderm cd38.L X. laevis Throughout NF stage 29 and 30 mesoderm head mesoderm Image source: Published Larger Image Printer Friendly View

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