Xenbase Image ID: 49485

Supplementary Figure 4: XtSulf-1 and XtSulf-2 are expressed in the migrating CNC and are flanked by FGF3 and FGF8 expression. Zygotic transcripts for XtSulf-1, XtSulf-2 , FGF3 and FGF8 are detected by whole mount in-situ hybridisation using DIG-AP labelled RNA in X. tropicalis embryos at NF stage 25 30 35 and 40, lateral views of embryos, anterior to the left. XtSulf-1 is expressed in the mandibular arch and somites throughout all tailbud stages, in the hyoid and branchial arch and pronephros from NF stage 25 till 40 in the primary heart field from NF stage 30 till stage 40. XtSulf-2 is expressed in the anterior mesendoderm, just ventral to the mandibular arch at NF stage 20 to 30, expression is then restricted to the gill at NF stage 40. Frontal vibratome sections show XtSulf-1 expression in the pharyngeal mesoderm of the anterior and posterior branchial, hyoid and mandibular arches, XtSulf-2 expression in the pharyngeal endoderm of the mandibular arch and anterior ectodermal cleft of the hyoid arch, FGF3 and FGF8 expression in the anterior endodermal pharyngeal pouch of the posterior and anterior branchial, hyoid and mandibular arches. Xenbase Curator notes: 1) bottom 2 panels, left side are labelled sox8 as are curated as such. 2) NF staging mis-identified in figure legend, and are curated as stage 22/23, 26, 28/29 and 40 respectively (left to right) - not 2,5 30, 35, and 40 as stated above) Image published in: Guiral EC et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue fgf3 fgf-3, INT-2, int-2, xfgf3 X. tropicalis Sometime during NF stage 22 to NF stage 23 brain hindbrain midbrain-hindbrain boundary circumblastoporal collar cranial neural crest mandibular crest branchial crest hyoid crest eye primordium tail bud fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. tropicalis Sometime during NF stage 22 to NF stage 23 tail bud hatching gland midbrain-hindbrain boundary cranial neural crest hyoid crest anterior branchial crest posterior branchial crest anterior dorsal lateral plate region sulf2 xtsulf2 X. tropicalis Sometime during NF stage 22 to NF stage 23 midbrain mandibular arch eye primordium anterior neural tube sox8 X. tropicalis Sometime during NF stage 22 to NF stage 23 midbrain tail bud anterior neural tube neural tube roof plate cranial neural crest mandibular crest hyoid crest branchial crest sulf1 XtSulf-1, XtSulf1 X. tropicalis Throughout NF stage 23 paraxial mesoderm presomitic mesoderm periocular region pronephric mesenchyme mandibular crest anterior neural tube posterior neural tube floor plate fgf3 fgf-3, INT-2, int-2, xfgf3 X. tropicalis Throughout NF stage 26 cranial neural crest mandibular crest eye anterior branchial crest posterior branchial crest brain tail bud midbrain-hindbrain boundary sulf2 xtsulf2 X. tropicalis Throughout NF stage 26 midbrain mandibular arch neural tube hindbrain head region endomesoderm anterior neural tube ventral blood island sulf1 XtSulf-1, XtSulf1 X. tropicalis Throughout NF stage 26 paraxial mesoderm cranial neural crest tail bud presomitic mesoderm intersomitic region pronephric mesenchyme periocular region neural tube anterior neural tube floor plate sox8 X. tropicalis Throughout NF stage 26 midbrain cranial neural crest neural tube tail bud hindbrain rhombomere mandibular crest hyoid crest anterior branchial crest posterior branchial crest ventral blood island fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. tropicalis Throughout NF stage 26 tail bud hatching gland brain midbrain-hindbrain boundary cranial neural crest hyoid crest anterior branchial crest posterior branchial crest anterior dorsal lateral plate region head region fgf3 fgf-3, INT-2, int-2, xfgf3 X. tropicalis Throughout NF stage 28 eye brain forebrain midbrain-hindbrain boundary tail tip hyoid crest visceral pouch mandibular crest sulf2 xtsulf2 X. tropicalis Throughout NF stage 28 midbrain head mandibular arch pineal gland endomesoderm anterior neural tube ventral blood island fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. tropicalis Sometime during NF stage 28 to NF stage 29 and 30 tail bud hatching gland midbrain-hindbrain boundary pharyngeal arch hyoid arch branchial arch intersomitic region sulf1 XtSulf-1, XtSulf1 X. tropicalis Throughout NF stage 28 to NF stage 29 and 30 mandibular arch neural crest eye somite neural tube branchial arch pronephric kidney hyoid arch primary heart field floor plate dorsal tail fin dorsal fin margin ventral tail fin presomitic mesoderm intersomitic region trigeminal ganglion fgf3 fgf-3, INT-2, int-2, xfgf3 X. tropicalis Throughout NF stage 40 head region visceral pouch visceral pouch 3 visceral pouch 4 fgf8 AIGF, FGF-8, fgf8a, fgf-8b, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. tropicalis Throughout NF stage 40 hatching gland head region visceral pouch visceral pouch 1 visceral pouch 3 visceral pouch 4 sulf2 xtsulf2 X. tropicalis Throughout NF stage 40 head visceral pouch visceral pouch 1 gill primordium branchial arch 1 sulf1 XtSulf-1, XtSulf1 X. tropicalis Throughout NF stage 40 head region branchial arch branchial arch 1 branchial arch 2 branchial arch 3 branchial arch 4 Image source: Published Larger Image Printer Friendly View

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