XB-IMG-26287
Xenbase Image ID: 26287
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Figure 2. Developmental expression pattern of XLhx9 compared to XPax6 and XLhx2 as determined by whole-mount in situ hybridization. A, B: XLhx9 expression is apparent in the future hindbrain (hb) and retina (ret) at stages 18 (A) and 25 (B). C, D: At stages 30 (C) and 33/34 (D), XLhx9 is expressed in the telencephalon (tel), dorsal diencephalon (di), mesencephalon (mes), and hindbrain. Inset in C: Enlarged view of diencephalon and mesencephalon; relative size of expression domains indicated with brackets. E: XPax6 expression in a stage-25 embryo for comparison to XLhx9. F-H: Vibratome retinal sections after XLhx9 in situ. At stage 25 (F), XLhx9 is expressed throughout the presumptive retinal pigmented epithelium (PRPE) and presumptive neural retina (PNR), but reduced or absent in the optic stalk (OS). By stage 30 (G), XLhx9 expression is confined to the proliferative ciliary margin zone (CMZ) of the retina (arrows). At stage 33/34 (H), XLhx9 is expressed throughout the inner nuclear layer (INL, arrowheads). I-L: In situ hybridizations against XLhx2 on stage matched siblings of embryos in A-D. At stage 18 (I) and 25 (J), XLhx2 is expressed strongly within the eye field, but has reduced or absent expression in the hindbrain as compared to XLhx9. Strong telencephalic expression is also obvious at stage 25 (J). K, L: XLhx2 is expressed within the telencephalon, diencephalon, pineal gland (pi), mesencephalon, and hindbrain at stages 30 (K) and 33/34 (L). Image published in: Atkinson-Leadbeater K et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.
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