Xenbase Image ID: 23758

Fig. 6. Results of Xenopus neural crest ablation and xId2 expression patterns. (A, B) xSlug expression in the neural crest was used as a guide (A) for stage 15 neural crest ablations (B, abl). (C, D) Whole-mount xId2 in situ hybridization (ISH) was carried out on stage 39 control and neural crest ablated embryos. (C) There is xId2 expression in both the inflow tract (IFT) (green arrow) and outflow tract (OFT) (red arrow) of the developing heart, as well as the otic vesicle (O), eye (E), and branchial arches (BA) in the control embryo. (D) In the ablated stage 39 embryo, there is lack of expression in the IFT (green arrowhead), OFT (red arrowhead), and reduced expression in the BA. (E–G) Control embryos underwent whole-mount in situ hybridization (ISH) for xId2 and were fixed at stage 37/38 for whole-mount analysis and at stage 39 for sectional analysis. (E) xId2 expression is located in the branchial arches (BA), eye (E), otic vesicle (O), and heart (H). (F, G) xId2 expression is located in the neural tube (NT), eye (E), outflow tract (OFT), pharynx (P), nerve ganglion (green arrows), and splanchnic mesoderm (SM, red arrows). Image published in: Martinsen BJ et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue id2.L xid2 X. laevis Sometime during NF stage 37 and 38 to NF stage 39 outflow tract eye heart otic vesicle branchial arch neural tube pharynx nerve ganglion splanchnic layer of lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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