Xenbase Image ID: 198932

Figure 1. Npr3, a target of Pax3 and Zic1, is expressed in developing NC and CP. (A) By ISH, npr3 is detected at the neural plate border at neurula stages (NF stage 1317), and persists in the NC (yellow arrowheads) and CP (purple arrowheads) forming regions (NF stage 2226). npr3 expression is maintained in most CP-derivatives at NF stage 32. (B) Dissected brain regions at NF stages 3034 show additional npr3 expression domains in the floor plate (green arrowheads), zona limitans intrathalamica (blue arrowheads) and pineal gland (orange arrowheads). Upper right shows a transverse section through the hindbrain of a NF stage 30 embryo highlighting the floor plate expression of npr3 (green arrowhead). The embryonic stages are indicated in the lower right corner of each panel. (C) Zic1 (Zic1MO) or Pax3 (Pax3MO) knockdown reduces npr3 expression at the NPB. Injection of a CoMO did not affect npr3 expression. The asterisks (*) mark the injected side, also visualized by the lineage tracer Red-gal. Dorsal views, anterior to top. (D) Frequency of the phenotypes. The number of embryos analyzed is indicated at the top of each bar. ***p<0.0005, [Chi-squared] test.(EJ) Developmental qRT-PCR expression profile of npr1 (E), npr2 (F), npr3 (G), nppa (H), nppb (I) and nppc (J) in Xenopus. NF developmental stages are indicated on the x-axis, values are normalized to ornithine decarboxylase (odc). A representative experiment is shown. Two additional biological replicates are shown in Figure S1 Image published in: Devotta A et al. (2023) Copyright © 2023. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue npr3.L npr-c, nprc X. laevis Throughout NF stage 13 neural plate border neuroectoderm npr3.L npr-c, nprc X. laevis Throughout NF stage 15 neural plate border neural crest neural groove cranial placode npr3.L npr-c, nprc X. laevis Throughout NF stage 17 neural plate border neural crest cranial placode neural groove neuroectoderm npr3.L npr-c, nprc X. laevis Throughout NF stage 22 cranial neural crest anterior placodal area brain neural tube cranial placode npr3.L npr-c, nprc X. laevis Throughout NF stage 26 cranial neural crest spinal cord forebrain cranial placode npr3.L npr-c, nprc X. laevis Throughout NF stage 32 lens placode epibranchial placode olfactory placode otic vesicle forebrain spinal cord midbrain npr3.L npr-c, nprc X. laevis Throughout NF stage 29 and 30 brain zona limitans intrathalamica pineal gland spinal cord floor plate npr3.L npr-c, nprc X. laevis Sometime during NF stage 32 to NF stage 33 and 34 brain pineal gland zona limitans intrathalamica spinal cord floor plate Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pax3 MO + NF15 (in situ hybridization) Fig. 1C Expression Phenotype absent npr3.L expression in neural crest decreased amount pax3.L expression in cranial placode Xla Wt + zic1 MO + NF15 (in situ hybridization) Fig. 1C Expression Phenotype absent npr3.L expression in cranial placode absent npr3.L expression in neural crest Larger Image Printer Friendly View

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