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XB-ART-59841
Elife 2023 May 10;12. doi: 10.7554/eLife.84036.
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Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor.

Devotta A , Juraver-Geslin H , Griffin C , Saint-Jeannet JP .


Abstract
Natriuretic peptide signaling has been implicated in a broad range of physiological processes, regulating blood volume and pressure, ventricular hypertrophy, fat metabolism, and long bone growth. Here, we describe a completely novel role for natriuretic peptide signaling in the control of neural crest (NC) and cranial placode (CP) progenitors formation. Among the components of this signaling pathway, we show that natriuretic peptide receptor 3 (Npr3) plays a pivotal role by differentially regulating two developmental programs through its dual function as clearance and signaling receptor. Using a combination of MO-based knockdowns, pharmacological inhibitors and rescue assays we demonstrate that Npr3 cooperate with guanylate cyclase natriuretic peptide receptor 1 (Npr1) and natriuretic peptides (Nppa/Nppc) to regulate NC and CP formation, pointing at a broad requirement of this signaling pathway in early embryogenesis. We propose that Npr3 acts as a clearance receptor to regulate local concentrations of natriuretic peptides for optimal cGMP production through Npr1 activation, and as a signaling receptor to control cAMP levels through inhibition of adenylyl cyclase. The intracellular modulation of these second messengers therefore participates in the segregation of NC and CP cell populations.

PubMed ID: 37162198
PMC ID: PMC10212560
Article link: Elife
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: eya1 foxi4 foxi4.2 krt12.4 npb nppa nppb nppc npr1 npr2 npr3 ostn pax3 six1 snai2 sox10 sox2 twist1 zic1
Antibodies: Npr1 Ab1 Npr2 Ab2 Npr3 Ab1 Npr3 Ab2
Morpholinos: nppa MO1 nppb MO1 nppc MO1 npr1 MO1 npr2 MO1 npr3 MO1 pax3 MO1 zic1 MO1

Phenotypes: Xla Wt + nppa MO (Fig 6 Ar1 B) [+]

Article Images: [+] show captions
References [+] :
Abdelalim, NPR-A regulates self-renewal and pluripotency of embryonic stem cells. 2011, Pubmed