Xenbase Image ID: 192517

Fig. 1. Lrp2 is expressed in the neuroepithelium and increased in constricting cells. (A-D) lrp2 mRNA (A) and protein (B-D) expression analyzed by in situ hybridization and immunofluorescence, respectively. Neurula stage (st.) embryos (frontal views, dorsal upwards). (A) Neural lrp2 expression. (B) Stage 15 forebrain region; Lrp2 is expressed in most cells (outlined by F-actin); single cells with high Lrp2 levels are located along the anterior rim of neural folds (NFs; arrowheads). (B′) Magnification of boxed region in B; increased Lrp2 levels are found in cells with small apical surface (circled in single channels B′1 and B′2). (C) LRP2 is detected throughout E8.5 anterior NFs, concentrated in areas undergoing apical constriction. Compare C′ (constricted cells in optic evagination) with C″ (dorsolateral cells with larger cell surface). ZO-1 marks cell boundaries. (D) STED imaging; LRP2 is condensed around neuroepithelial primary cilia (ARL13b+) at E9.5. Scale bars: 10†µm in B′,C′,C″; 1†µm in D. Image published in: Kowalczyk I et al. (2021) Copyright © 2021. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue lrp2.L dbs, gp330, megalin X. laevis Sometime during NF stage 13 to NF stage 15 neural plate neuroectoderm anterior neuroectoderm anterior neural fold neural fold actb.L beta actin, beta-actin, g-actin X. laevis Throughout NF stage 15 neural plate neural fold actin filament Image source: Published Larger Image Printer Friendly View

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