XB-IMG-175015
Xenbase Image ID: 175015
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Fig. 8. Detection of xBGP mRNA by RT-PCR. Total RNA was extracted from whole froglet (65) and tissues (liver, heart, bone, kidney and muscle) of Xenopus and used to amplify xBGP mRNA by RT-PCR, using two specific primers (XBGP8F and XBGP4R), as described in Section 2. PCR products were size fractionated by agarose gel electrophoresis, transferred to a nylon membrane and hybridized with a 32P-labelled xBGP cDNA (bottom panel). To check for RNA integrity, the same RT reactions were used to amplify xODC mRNA using two specific primers based on the published sequence (top panel).
Image published in: Viegas CS et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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