XB-IMG-173784
Xenbase Image ID: 173784
|
Fig. 1. Temporal and spatial expression pattern of Fam46a [tent5a] in Xenopus.
(A) RT-PCR analysis of Fam46a expression. The expression of Fam46a is faintly
detected from the maternal stage to stage 9, and increased from stage 10, similar to
that of Zic1 (NPB), Vent1, Vent2 (BMP target gene), and Chordin (BMP antagonist).
The expression of PPE marker genes, Six1 and Eya1, increased soon after the onset
of Fam46a expression. ODC was used as a control. (B-E) Whole-mount in situ
hybridization (WISH) was performed with a Fam46a (B, B’) and Six1 (C, C’) probe.
The anterior view (B, C); the lateral view (D, E); the hemi-section (B’, C’, D’, D”, E’).
Development • Accepted manuscript
The stage of the embryo was shown in each panel. PPE, pre-placodal ectoderm; Op,
optic vesicle; Ot, otic vesicle; Le, lens; Br, branchial arch mesenchyme; RPE, retinal
pigment epithelium. (F) HeLa cells were transfected with GFP-Fam46a and cultured
for 24 hours. Nuclei were stained with NUCLEAR-ID Red. Scale bars represent 20 μ
m. Image published in: Watanabe T et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Image source: Published Larger Image Printer Friendly View |