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XB-IMG-171552

Xenbase Image ID: 171552


Fig. 1 LARP6 morphants have neural tube closure defects. a Temporal expression profiles of LARP6 gene. b Spatial expression profiles of LARP6 gene. c LARP6 MO (L6MO) but not control MO (conMO) depleted X. laevis LARP6 protein (xlL6-2HA) in late neurula stage embryos. conMO or L6MO was injected with xlL6-2HA mRNA, and LARP6 was detected by immunoblotting with anti-HA antibody. d Human LARP6 (HA-hL6) was not knocked down by L6MO in stage 10 or 20 embryos. e Depletion of LARP6 resulted in neural tube closure defects. conMO, L6MO (15 ng/blastomere) and/or HA-hL6 mRNA (250 pg/blastomere) were injected into two dorsal blastomeres of 4-cell stage embryos (between animal pole and marginal zone). Images are dorsal views of late neurula stage embryos. f The summary of rescue experiments: the C-terminus of LARP6 is important for neural tube closure. LAM La motif, RRM RNA recognition motif, SUZC SUZ-C motif. n indicates number of embryos examined. 5′SL binding shows the interaction between LARP6 and the 5′ stem-loop region of collagen RNA. F phenylalanine, A alanine, E glutamic acid, a anterior, p posterior, b brain, e eye. ODC and β-actin were used as a loading control

Image published in: Manojlovic Z et al. (2017)

© The Author(s) 2017. Creative Commons Attribution license

GeneSynonymsSpeciesStage(s)Tissue
larp6.LX. laevisThroughout NF stage 9animal hemisphere
larp6.LX. laevisThroughout NF stage 14neural plate
pre-chordal neural plate
chordal neural plate
larp6.LX. laevisThroughout NF stage 20neural tube
posterior neural tube
anterior neural tube
neural plate
larp6.LX. laevisThroughout NF stage 25neural tube
posterior neural tube
anterior neural tube
eye
optic vesicle
larp6.LX. laevisThroughout NF stage 29 and 30central nervous system
brain
spinal cord
pharyngeal arch
eye

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