Xenbase Image ID: 157333

Fig. 5. Localization of XL43 and XL75 mRNA in early Xenopus embryos by whole-mount in situ hybridization. (A) Antisense riboprobes. 1: Lateral view of a yolk plug stage embryo (St. 11.5) hybridized with XL75 probe. The dorsal ectodermic region is essentially labelled and the yolk plug is totally negative. 2: Dorsal view of a neurula (St. 18) labelled with XL75 probe. XL75 mRNA is localized in the neurectoderm and neural plate. 3,4: Lateral view of tailbud stages (St. 32–33) hybridized with XL43 probe (3) and XL75 (4) probe. In each case, mRNA is present essentially in mesodermic tissues, in the head, somites and notochord. (B) Sense probes. Lateral view of a yolk plug stage embryo (St. 11.5) hybridized with XL75 probe (1), a neurula (St. 19) labelled with XL43 probe (2), and a tailbud stage (St. 33) hybridized with XL75 probe (3). Only background staining is observed. Image published in: Perrin K and Lacroix JC (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue trim7.L gnip, rnf90, XL75 X. laevis Sometime during NF stage 11 to NF stage 11.5 dorsal ectoderm trim7.L gnip, rnf90, XL75 X. laevis Throughout NF stage 18 neuroectoderm neural plate trim7.L gnip, rnf90, XL75 X. laevis Throughout NF stage 32 notochord somite head spinal cord Image source: Published Larger Image Printer Friendly View

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