XB-IMG-153797
Xenbase Image ID: 153797
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Figure 4—figure supplement 3.
Expression of targets with dynamic/transient expression pattern in placodes in whole-mount Xenopus embryos.
Expression dynamics for each target are shown across a range of developmental stages: A1–E1 show expression in neural plate stage embryos, A2–E2 show early tail bud stage embryos and A3–E3 show late tail bud stage embryos. (A) Expression of PPE marker gene Six1 is shown as reference for placodal domains (for details see Pandur and Moody, 2000; Schlosser and Ahrens, 2004). (B) Hes2 is expressed very strongly in a broad region corresponding to the posterior placodal domain including the prospective otic and lateral line placodes, as well as weakly in a scattered subset of neuroectodermal cells (B1; asterisk) at neural plate stages (see Figure 4—figure supplement 5 for section). Expression is later restricted to the otic vesicle and a new expression domain becomes established in the developing retina at early and late tail bud stages (B2 and B3; arrowhead). (C) During neural plate stages (C1), Hes8 is expressed in the developing profundal and trigeminal placodes as well as in the anterior placodal region, the anterior neural plate (C1 ; asterisk) and the primary neurons (motor neurons, intermediary neurons and sensory neurons) of the posterior neural plate (C1; arrowheads; see Figure 4— figure supplement 5 for section). In early tail bud stages (C2) trigeminal expression is lost and replaced by expression in the otic vesicle, as well as lateral line, epibranchial and olfactory placodes. Throughout late tail bud stages (C3), expression is maintained in these regions and the brain (C3; cross) and is initiated in the retina (C3; diamond). (D) During neural plate stages (D1), Hes9 is expressed in the developing profundal and trigeminal placodes as well as in the anterior placodal region, the anterior neural plate (D1; asterisk) and the primary neurons (motor neurons, intermediary neurons and sensory neurons) of the posterior neural plate (D1; arrowheads; see Figure 4—figure supplement 5 for section). In early tail bud stages (D2) trigeminal expression is lost but expression is apparent in the olfactory placodes, as well as in the otic and lateral line placodes and retina (D2; diamond). In late tail bud stages (D3) Hes9 is expressed broadly thoughout the brain (D3 ; cross), and is maintained in the lateral line and olfactory placodes as well as the otic vesicle. (E) During neural plate stages (E1), Mab21l2b is expressed in the prospective lens placode, as well as in the eye field (prospective retina) of the forebrain (E1; asterisk) and the prospective midbrain (E1; arrowhead). At early tail bud stages expression in the lens and midbrain is maintained and its expression becomes apparent in the hindbrain (E2; diamond). In late tail bud stages Mab21l2b is additionally prominently expressed in migrating neural crest cells (E3 ; Nc). (F) During neural plate stages Sox21 is expressed broadly throughout the anterior neural plate (F1; asterisk). At early tail bud stages (F2), this expression becomes confined to the forebrain (asterisk in F2) and midbrain-hindbrain boundary (arrowhead in F2) and is maintained into late tail bud stages, (F3). In late tail bud stages, Sox21 is also expressed in the olfactory placode, otic vesicle and becomes up-regulated in the hindbrain (F3; diamond). Yellow arrows mark placodal expression. Dotted lines in B1–D1 indicate levels of sections shown in Figure 4—figure supplement 5. Abbreviations: pA: anterior placodal region; pAD: anterior lateral line placode; pE: epibranchial placode; pLl: lateral line placodes; L: lens; pL: lens placode; pM: middle lateral line placode; pO: olfactory placode; vOt: otic vesicle; pP: posterior placodal region; pPrV: profundal/trigeminal placodes. Image published in: Riddiford N and Schlosser G (2016) © 2016, Riddiford et al. Creative Commons Attribution license
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