XB-IMG-135482
Xenbase Image ID: 135482
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Figure 4.
PR72 is required for cell morphogenetic movements during gastrulation. (A) RT-PCR analysis of X. tropicalis total RNA from different developmental stages. XPR72 and Xnkd are expressed maternally (stage 7) and throughout early development (stages 10, 18, 22, and 30). (-RT) RT-PCR without reverse transcriptase. ODC1 expression was used as a loading control. (Bottom panel) Whole-mount in situ hybridization for XPR72 in embryos showing predominant staining of somites. (B) Two sets of three nonoverlapping PR72 MOs (1, 4, 5 or 1, 2, 3) both show similar phenotypes (bottom two panels) when injected dorsally at the four-cell stage at equal amounts (10 ng total) compared to control MO-injected (top panel). Phenotypes include defective cell morphogenetic movements during gastrulation, defect in eye development, incomplete neural tube closure (arrows), and incomplete closure of the blastopore during late gastrulation (arrowheads). (C) Injection of PR72 MO in either the left half (middle panel) or right half (right panel) results in a bent axis (black arrow) and underdevelopment of the eye (white arrow) compared to control MO-injected embryos (left panel). (D) Coinjection of 20 pg of human PR72 RNA rescues the developmental defect (graph). Image published in: Creyghton MP et al. (2005) Copyright © 2005. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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