Xenbase Image ID: 134535

FIGURE 1. Temporal and spatial expression profile of XH2AX during embryogenesis. A, RT-PCR analysis of temporal mRNA expression of XH2AX. Developmental stages are indicated above each lane. Histone H4 served as a loading control. −RT, control reaction without reverse transcriptase. B–I, whole-mount in situ hybridization showing the spatial expression of XH2AX during early Xenopus development. B and C, blastula stage: animal hemisphere view (B) and vegetal hemisphere view (C); D and E, gastrula stage: animal hemisphere view (D) and vegetal hemisphere view (E); F, neurula stage: anterior view with posterior right; G and H, tail bud stage: lateral view with anterior left (G) and dorsal view with anterior left (H); I, stages 33–34. The black lines represent the angle of sectioning for J–L. J–L, transverse section through a stage 33–34 embryo stained by whole-mount in situ hybridization for XH2AX mRNA. ba, branchial arches; br, brain; ey, eye; hg, hatching gland; mb, midbrain; ov, otic vesicle; so, somite. Image published in: Lee SY et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue h2ax.S h2afx, XH2AX X. laevis Sometime during NF stage 7 to NF stage 33 and 34 animal cap eye eye primordium optic vesicle brain forebrain midbrain hindbrain animal hemisphere Image source: Published Larger Image Printer Friendly View

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