Xenbase Image ID: 123456

Figure 7. Internal perfusion of TEA delays deactivation and slow inactivation in Shaker IR. (A) Elements of the analysis of the effect of internal TEA on the tails of currents measured at −110 mV. As examples, the first and last traces of the experiment in Fig. 5 are shown. Current deactivations were fit to a single exponential function, and the time constant is shown on top of each trace (discontinuous lines). The amplitudes ao and a are extrapolations of the curves fit to the peak of the preceding capacitive transient, in which a is the amplitude of a given trace and ao is the amplitude of the first one. The ratio a/ao is the unblocked fraction at −110 mV. (B) Plot of the deactivation rate as a function of the unblocked fraction of currents for five different oocytes. Continuous lines are the linear regressions used to estimate the values of α and β (see main text). (C) Elements of the analysis of the effect of TEA on the ionic currents measured at 0 mV. Fitted single exponential functions are shown as discontinuous lines on top of two example traces with the times constants shown, while a and ao have the same meaning as in A. (D) Plot of the slow inactivation rate as a function of the unblocked fraction of currents for the five oocytes shown in B. Identical symbols in B and C correspond to data from the same cell. Continuous lines are linear regressions to estimate the values of TEA-dependent inactivation constant. Image published in: González-Pérez V et al. (2008) © 2008 González-Pérez et al. Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

Return to previous page