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XB-PHENO-25962

Xtr Wt + otx2 MO + crx MO + gsc MO + lhx1 MO, Yasuoka Y et al. (2014)

Experiment + Assay: Xtr Wt + otx2 MO + crx MO + gsc MO + lhx1 MO + NF31-32 (whole-mount microscopy)
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection lhx1 MO1 lhx1
 NF stage 2 (2-cell) animal pole
morpholino injection otx2 MO3 otx2
 NF stage 2 (2-cell) animal pole
morpholino injection crx MO1 crx
 NF stage 2 (2-cell) animal pole
morpholino injection gsc MO1 gsc
 NF stage 2 (2-cell) animal pole
Conditions:
Assay Type Assay Stages Antibody
whole-mount microscopy NF stage 31 to NF stage 32  
Anatomical Phenotypes: abnormal forebrain , abnormal foregut morphology , abnormal head morphology , absent eye , absent optic stalk , decreased size of the forebrain , decreased size of the midbrain-hindbrain boundary
Expression Phenotypes (1):
absent eomes expression in optic stalk
Source: Fig. 1 b row2
Article: XB-ART-49342, PubMed