Xenbase Image ID: 80348

FIG. 3. Noelin-1 and -2 expression during development. Whole mount in situ hybridization with a probe to the Z exon reveals the distribution of Noelin-1 and Noelin-2 transcripts in the neural tube and cranial ganglia. All embryos are oriented with anterior to the right. A: Earliest expression is seen after neural tube closure in the spinal cord and trigeminal ganglia at stage 22. B: By stage 27 cells in the olfactory placodes and in the trigeminal (V) and geniculate (VII) ganglia are strongly reactive. Punctate staining is visible in the pineal gland and spinal cord. C: A stage 33 embryo shows increasing signal in the Vth and VIIth ganglia, olfactory pits, pineal gland and spinal cord. A population of cells in the retina is also positive for Noelin-1 and -2. D: By stage 35/36 the IXth and Xth ganglia are also positive for the transcripts. A small number of cells in the branchial arches express the Z exon (arrowhead). E-G: Dorsal views of embryos in A-C. E: Stage 22 embryo shows staining in the Vth ganglia and in the spinal cord. Transcripts are not detected in the brain at this stage. The black arrow marks the anterior extent of Noelin-1 and -2 signal in the neural tube. F: By stage 27, spinal cord staining extends into the brain and up to the level of the midbrain. The pineal gland and olfactory placodes are visible. G: At stage 33 the signal is increasingly intense in the cranial ganglia, spinal cord, and more rostrally into the forebrain. e, eye; hb, hindbrain; o, olfactory placode/pit; p, pineal gland; sc, spinal cord; V, trigeminal; VII, geniculate; IX, glossopharyngeal; X, vagal; asterisks mark the general position of otic vesicles. Brown color is due to melanocytes in the skin or retinal pigmented epithelium in the eye. Image published in: Moreno TA and Bronner-Fraser M (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue olfm1.S noelin-1 X. laevis Throughout NF stage 22 trigeminal placode posterior neural tube neural tube spinal cord olfm1.S noelin-1 X. laevis Throughout NF stage 27 spinal cord hindbrain brain pineal gland olfactory placode olfm1.S noelin-1 X. laevis Throughout NF stage 33 and 34 spinal cord trigeminal nerve facial nerve olfactory bulb pineal gland central nervous system eye cranial nerve forebrain brain hindbrain olfm1.S noelin-1 X. laevis Throughout NF stage 35 and 36 spinal cord olfactory bulb central nervous system vagus nerve forebrain midbrain brain hindbrain glossopharyngeal nerve olfm2.L noe2, noelin-2 X. laevis Throughout NF stage 22 to NF stage 35 and 36 spinal cord trigeminal nerve facial nerve trigeminal placode olfactory placode olfactory bulb pineal gland central nervous system eye cranial nerve vagus nerve forebrain midbrain brain hindbrain neural tube glossopharyngeal nerve posterior neural tube Image source: Published Larger Image Printer Friendly View

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