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XB-IMG-192462

Xenbase Image ID: 192462


Figure 3—figure supplement 1. Validation of Bcat-MO embryos. (A) Immunostaining of Tg(WntREs:dEGFP)Vlem Xenopus tropicalis gastrula embryos show the loss of nuclear Bcat and GFP expression from the Wnt-reporter transgene in Bcat-MO embryos demonstrate effective knockdown. (B–C) Co-injection of RNA encoding a human S37A-stabilized Bcat recues the Bcat-MO-ventralized phenotype. In situ hybridization of gastrula (B) and tailbud embryos (C). In Bcat-MO gastrula, fst and admp are downregulated in the dorsal organizer, whereas wnt8a and ventx3.1 are upregulated consistent with a ventralized phenotype. (C) In situ hybridization of markers for the liver (nr1h5), stomach, lung, intestine and pharynx (osr1 and islet1) show that co-injection of human S37A-stabilized Bcat RNA rescues the ventralized phenotype and disrupted gut development in Bcat-MO tailbud embryos.

Image published in: Mukherjee S et al. (2020)

© 2020, Mukherjee et al. Creative Commons Attribution license

GeneSynonymsSpeciesStage(s)Tissue
fstfoll, follistatin, xfsX. tropicalisThroughout NF stage 10.5involuting marginal zone
involuted dorsal mesoderm
admpX. tropicalisThroughout NF stage 10.5endoderm
dorsal endomesoderm
involuted dorsal mesoderm
axial mesoderm
ventx3vent-3, ventx3.1X. tropicalisThroughout NF stage 10.5ventral endoderm
ventro-lateral marginal zone
wnt8awnt-8, wnt8, Xwnt-8, Xwnt8X. tropicalisThroughout NF stage 10.5ventro-lateral marginal zone
involuted ventral mesoderm
nr1h5for1X. tropicalisThroughout NF stage 35 and 36liver
osr1odd1X. tropicalisThroughout NF stage 35 and 36lung
stomach
cement gland
isl1islet-1, islet1, Xisl-1, Xislet-1X. tropicalisThroughout NF stage 35 and 36pharynx
stomach
intestine
spinal cord
pharyngeal region
eye
profundus ganglion
midbrain

Image source: Published

Experiment + Assay Source Phenotypes and Disease
Xtr Wt + ctnnb1 MO + NF10.5 (immunohistochemistry) Fig.3.S1.A
Anatomical Phenotype
abnormal Wnt signaling pathway
Expression Phenotype
decreased amount ctnnb1 expression in nucleus
Xtr Wt + ctnnb1 MO + NF10.5 (in situ hybridization) Fig.3.S1.B
Expression Phenotype
decreased amount admp expression in axial mesoderm
decreased amount admp expression in dorsal endomesoderm
decreased amount admp expression in endoderm
absent fst expression in dorsal involuting marginal zone
absent fst expression in involuted dorsal mesoderm
increased amount ventx3.1 expression in animal cap
increased amount ventx3.1 expression in blastocoel floor
increased amount ventx3.1 expression in endoderm
increased amount wnt8a expression in blastocoel floor
increased amount wnt8a expression in ectoderm
increased amount wnt8a expression in endoderm
increased amount wnt8a expression in the animal cap
increased amount wnt8a expression in ventral marginal zone
Xtr Wt + ctnnb1 MO + NF35/36 (in situ hybridization) Fig.3.S1.C
Anatomical Phenotype
wholly ventralized embryo
Expression Phenotype
absent isl1 expression in embryo
absent nr1h5 expression in embryo
absent osr1 expression in embryo

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