XB-IMG-146239
Xenbase Image ID: 146239
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Figure 8. The Temporal and Spatial Expression of FRL7 and FRLP
Genes during Embryogenesis
(A) Temporal expression. RNA was isolated from unfertilized eggs and
the indicated stages of embryos. The expression of the FRLl and FRLP
genes was assayed by RT-PCR. Ornithine decarboxylase (ODC) is
present as an internal control.
(B) Spatial expression. Stage 10 embryos were dissected into the dorsal
half (Dr) and the ventral half (Vn), or into the vegetal half including
the marginal zone (Vg) and the animal half (An), and RNA was isolated
from these tissues or from stage 10 embryos (Em). The expression
of the FRLl gene was assayed by RT-PCR. goosecoid (gsc) is known
to be expressed in the dorsal marginal zone (Cho et al., 1991). EFlu
is a ubquitously expressed control (Krieg et al., 1989).
(C) Whole-mount in situ hybridization in the tailbud stage embryo of
FRL2 RNA. Purple staining indicates the localization of the antisense
FRL2 probe. Image published in: Kinoshita N et al. (1995) Copyright © 1995. Image reproduced with permission of the Publisher, Elsevier B. V.
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