Xenbase Image ID: 135112

Figure 1. LPAR2 signaling is required for in vivo NC migration. (A) In situ hybridization for Foxd3 and LPAR2. (B) In situ hybridization for Twist and LPAR2. Asterisks indicate eyes. (C) Control Morpholino coinjected with membrane-mCherry into animal caps expressing 5′UTR-LPAR2GFP. (D) LPAR2 Morpholino coinjected with membrane-mCherry into animal caps expressing 5′UTR-LPAR2GFP. Note the absence of LPAR2-GFP fluorescence in the cell injected with LPAR2MO. (E) Frontal view of embryos injected with Control MO (CMO) or LPAR2MO, Foxd3 in situ hybridization. Asterisks, eyes; Inj., injected side; red lines, normal migration in control side. (F) LPAR2MO-injected embryos have NC migration defects (41 embryos were analyzed). Student’s t test (two-tailed): ***, P < 0.005; errors bars indicate SD. (G) Grafting procedure. (H) Graft of control (left) or LPAR2MO (middle) or LPAR1MO cells (right) labeled with FDx (green) into host embryos. (I) Graft of control NC cells labeled with rhodamine-dextran (RDx, red) into LPAR2MO host (FDx, green). Bars: (A, B, E, H, and I) 0.5 mm; (C and D) 100 µm. Image published in: Kuriyama S et al. (2014) © 2014 Kuriyama et al. Creative Commons Attribution-NonCommercial-ShareAlike license Gene Synonyms Species Stage(s) Tissue lpar2.S lpa2 X. laevis Throughout NF stage 16 neural crest cranial neural crest migratory neural crest cell lpar2.S lpa2 X. laevis Throughout NF stage 25 cranial neural crest mandibular crest anterior branchial crest twist1.S twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 25 pharyngeal arch cranial neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 16 cranial neural crest migratory neural crest cell foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 23 cranial neural crest migratory neural crest cell Image source: Published Larger Image Printer Friendly View

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