Fig. S3 B

Yasuoka Y et al. (2014)

Xla Wt + tle1 + gsc + otx2 + ssbp3 + ldb1 + lhx1 mRNA

NF9 (RT-PCR)

Expression Phenotype
decreased amount actc1.L expression in embryo increased amount chrd.1.L expression in the embryo increased amount gsc.L expression in the embryo increased amount cer1.L expression in embryo increased amount tbxt.L expression in embryo

Fig. 1 b row2

Yasuoka Y et al. (2014)

Xtr Wt + otx2 MO + crx MO + gsc MO + lhx1 MO

NF31-32 (whole-mount microscopy)

Anatomical Phenotype
abnormal forebrain abnormal foregut morphology abnormal head morphology absent eye absent optic stalk decreased size of the forebrain decreased size of the midbrain-hindbrain boundary

Expression Phenotype
absent eomes expression in optic stalk

fig.S7

Rankin SA et al. (2011)

Xla Wt + lhx1 MO + otx2 MO

NF10.5 (in situ hybridization)

Expression Phenotype
decreased amount chrd.1.L expression in involuted dorsal mesoderm decreased amount gsc.L expression in involuted dorsal mesoderm decreased amount hhex.L expression in dorsal endomesoderm increased amount chrd.1.L expression in involuted dorsal mesoderm mislocalised chrd.1.L expression in involuted dorsal mesoderm

fig.7.c

Rankin SA et al. (2011)

Xla Wt + lhx1 MO + otx2 MO

NF11 (in situ hybridization)

Expression Phenotype
decreased amount gsc.L expression in dorsal endomesoderm decreased amount hhex.L expression in dorsal endoderm

fig.7.c

Rankin SA et al. (2011)

Xla Wt + lhx1 + otx2

NF11 (in situ hybridization)

Expression Phenotype
increased amount gsc.L expression in dorsal endomesoderm increased amount gsc.L expression in ventral endoderm increased amount hhex.L expression in dorsal endoderm increased amount hhex.L expression in ventral endoderm increased amount otx2.L expression in ventral endoderm mislocalised gsc.L expression in ventral endoderm mislocalised hhex.L expression in ventral endoderm

fig.7.c

Rankin SA et al. (2011)

Xla Wt + lhx1 + otx2 + sia1 MO + sia2 MO

NF11 (in situ hybridization)

Expression Phenotype
decreased amount hhex.L expression in dorsal endoderm increased amount gsc.L expression in dorsal endomesoderm

fig.S7

Rankin SA et al. (2011)

Xla Wt + otx2 MO

NF10.5 (in situ hybridization)

Expression Phenotype
decreased amount chrd.1.L expression in involuted dorsal mesoderm decreased amount gsc.L expression in involuted dorsal mesoderm decreased amount hhex.L expression in dorsal endomesoderm increased amount chrd.1.L expression in involuted dorsal mesoderm mislocalised chrd.1.L expression in involuted dorsal mesoderm

fig.2.c

Carron C et al. (2005)

Xla Wt + otx2

NF10.5 (in situ hybridization)

Expression Phenotype
decreased amount tbxt.S expression in upper blastopore lip

fig.2.f

Carron C et al. (2005)

Xla Wt + otx2

NF10.5 (in situ hybridization)

Expression Phenotype
decreased amount wnt11b.L expression in upper blastopore lip

fig.2.o

Carron C et al. (2005)

Xla Wt + otx2

NF12.5 (in situ hybridization)

Anatomical Phenotype
decreased size of the notochord

Expression Phenotype
decreased amount tbxt.S expression in notochord