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Fig. 2. Whole-mount in situ hybridisation analysis of Xenopus POX2 (A–H,L), LURP-1 (I,J) and L-plastin (K) expression. Right-lateral views of stage 19 (A), 24 (C) and 27 (E) embryos showing XPOX2-expressing cells. (B,D,F) Ventral views of the same embryos depicted in (A,C,E). (G) Higher magnification image of the stage 27 embryo depicted in (F) to illustrate the streams of XPOX2-expressing cells that emanate from a focal point (red arrow) located at the ventral midline within the heart-forming region. (H) Right-lateral view of a stage 30 embryo. (I,J) Left-lateral views of embryos at stages 24 and 36, respectively, showing cells that express XLURP-1. (K,L) Right-lateral view of the posterior trunk of a stage 35 tadpole after sequential, double whole-mount in situ hybridisation staining to reveal first (K), Xenopus L-plastin (pale blue) and second (L), POX2 (magenta) expression. The co-localisation of the two chromogenic reagents results in a dark blue colour (L). A, anterior; P, posterior.

Image published in: Smith SJ et al. (2002)

Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
mpo.Lmpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2X. laevisThroughout NF stage 19lateral plate mesoderm
ventral mesoderm
mpo.Lmpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2X. laevisThroughout NF stage 24anterior ventral blood island
hemangioblast
myeloid cell
slurp1l.Slurp1, Xlurp, XLURP-1X. laevisThroughout NF stage 24ventral blood island
anterior ventral blood island
hemangioblast
mpo.Lmpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2X. laevisThroughout NF stage 27anterior ventral blood island
hemangioblast
myeloid cell
mpo.Lmpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2X. laevisThroughout NF stage 29 and 30anterior ventral blood island
myeloid cell
slurp1l.Slurp1, Xlurp, XLURP-1X. laevisThroughout NF stage 35 and 36proctodeum
foregut
myeloid cell
post-anal gut
lcp1.Scp64, lc64p, lpl, l-plastin, pls2X. laevisThroughout NF stage 35 and 36myeloid cell
mpo.Lmpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2X. laevisThroughout NF stage 35 and 36myeloid cell

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