Larger Image Figure 5. Ssbp2 depletion reduces the expression domain of proximal and distal tubule markers. 8-cell stage Xenopus embryos were injected into a single V2 blastomere as indicated. The uninjected contralateral side was used as an internal control. (ae) WISH for pax2 in stage 32 embryos. *Indicate the position of the nephrostomes. (a) Uninjected embryo (8% affected, n=37, N=3). (b, c) St-MO 15 ng injected embryo (23% affected, n=40, N=2). (d, e) ssbp2-MO 15 ng injected embryo (66% affected, n=51, N=3). (fj) WISH for slc5a1 in stage 39 embryos. (f) Uninjected embryo (4% affected, n=47, N=2). (g, h) St-MO 15 ng injected embryo (19% affected, n=39, N=2). (i, j) ssbp2-MO 15 ng injected embryo (50% affected, n=34, N=2). (ko) WISH for clcnkb in stage 39 embryos. (k) Uninjected embryo (3% affected, n=35, N=2). (l, m) St-MO 15 ng injected embryo (23% affected, n=41, N=2). (n, o) ssbp2-MO 15 ng injected embryo (49% affected, n=33, N=2). Magnifications of the pronephric tubules enclosed by the black squares are shown in the left-bottom corner. Statistical significance was evaluated using Chi-square test (****p<0.0001; **p<0.01). *, Represent the comparison to the uninjected and the St-MO injected groups, respectively. Representative embryos are shown. Image published in: Cervino AS et al. (2023) Copyright © 2023. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 32 pronephric duct pronephric nephrostome midbrain-hindbrain boundary hindbrain eye optic stalk slc5a1.L sglt-1, SGLT1, slc5a1.1 X. laevis Throughout NF stage 39 pronephric kidney proximal tubule clcnkb.L ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k X. laevis Throughout NF stage 39 pronephric kidney distal tubule Image source: Published Experiment + Assay Source Phenotypes and Disease Xla WT + ssbp2 MO + NF32 (in situ hybridization) Fig. 5 e, j, o Anatomical Phenotype abnormal pronephric tubule morphology decreased size of the distal tubule decreased size of the pronephric tubule decreased size of the proximal tubule Expression Phenotype decreased amount clcnkb.L expression in pronephric tubule decreased amount pax2.L expression in pronephric tubule decreased amount pax2.L expression in proximal tubule decreased amount slc5a1.L expression in proximal tubule Permanent Image Page Printer Friendly View XB-IMG-197631