Larger Image Figure 4. In situ hybridization to sections of Xenopus embryos. [A] Dark-field illumination shows hybridization of NC-11 throughout the notochord from the anterior to posterior region of a stage 25 embryo (see £). [B] Dark-field illumination shows hybridization to the notochord in a stage 25 embryo. (C) Bright-field illumination of the section shown in B. (N) Notochord; (NT) neural tube. (D) Dark-field illumination of a stage 14 embryo shows hybridization to the notochord. (£) Plane of sections shown in A and B. Although the embryos are at different stages, the plane of section in D is the same as that in B. Image published in: LaFlamme SE et al. (1988) Copyright © 1988. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article.
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