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XB-ART-60586
STAR Protoc 2024 Mar 15;51:102895. doi: 10.1016/j.xpro.2024.102895.
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Protocol for tail vein injection in Xenopus tropicalis tadpoles.

Patel JH , Angell Swearer A , Kakebeen AD , Loh LR , Wills AE .


Abstract
Functional studies in post-embryonic Xenopus tadpoles are challenging because embryonic perturbations often lead to developmental consequences, such as lethality. Here, we describe a high-throughput protocol for tail vein injection to introduce fluorescent tracers into tadpoles, which we have previously used to effectively inject morpholinos and molecular antagonists. We describe steps for safely positioning tadpoles onto agarose double-coated plates, draining media, injecting into the ventral tail vein, rehydrating plates, and sorting tadpoles by fluorescence with minimal injury for high-throughput experiments. For complete details on the use and execution of this protocol, please refer to Kakebeen et al.,1 Patel et al.,2 and Patel et al.,3.

PubMed ID: 38367232
PMC ID: PMC10882117
Article link: STAR Protoc


Species referenced: Xenopus tropicalis


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