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STAR Protoc
2024 Mar 15;51:102895. doi: 10.1016/j.xpro.2024.102895.
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Protocol for tailvein injection in Xenopus tropicalis tadpoles.
Patel JH
,
Angell Swearer A
,
Kakebeen AD
,
Loh LR
,
Wills AE
.
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Functional studies in post-embryonic Xenopus tadpoles are challenging because embryonic perturbations often lead to developmental consequences, such as lethality. Here, we describe a high-throughput protocol for tailvein injection to introduce fluorescent tracers into tadpoles, which we have previously used to effectively inject morpholinos and molecular antagonists. We describe steps for safely positioning tadpoles onto agarose double-coated plates, draining media, injecting into the ventraltailvein, rehydrating plates, and sorting tadpoles by fluorescence with minimal injury for high-throughput experiments. For complete details on the use and execution of this protocol, please refer to Kakebeen et al.,1 Patel et al.,2 and Patel et al.,3.
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