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Dev Biol 2014 Feb 15;3862:473-83. doi: 10.1016/j.ydbio.2013.12.011.
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Identification of Pax3 and Zic1 targets in the developing neural crest.

Bae CJ , Park BY , Lee YH , Tobias JW , Hong CS , Saint-Jeannet JP .

The neural crest (NC) is a multipotent population of migratory cells unique to the vertebrate embryo, contributing to the development of multiple organ systems. Transcription factors pax3 and zic1 are among the earliest genes activated in NC progenitors, and they are both necessary and sufficient to promote NC fate. In order to further characterize the function of these transcription factors during NC development we have used hormone inducible fusion proteins in a Xenopus animal cap assay, and DNA microarray to identify downstream targets of Pax3 and Zic1. Here we present the results of this screen and the initial validation of these targets using quantitative RT-PCR, in situ hybridization and morpholinos-mediated knockdown. Among the targets identified we found several well-characterized NC-specific genes, including snail2, foxd3, gbx2, twist, sox8 and sox9, which validate our approach. We also obtained several factors with no known function in Xenopus NC, which represent novel regulators of NC fate. The comprehensive characterization of Pax3 and Zic1 targets function in the NC gene regulatory network, are essential to understanding the mechanisms regulating the emergence of this important cell population.

PubMed ID: 24360908
PMC ID: PMC3933997
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ak3 astl2c astl3a.1 chrd fgf8 foxd3 gbx2 gbx2.2 lmx1b mfap2 npr3 odc1 p2ry4 pax3 pdgfra pmp22 pnhd rassf10 six1 snai2 sox2 sox8 sox9 tspan18 twist1 zic1
Morpholinos: pax3 MO1 wnt8a MO1 zic1 MO1

Article Images: [+] show captions
References [+] :
Bajard, A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. 2006, Pubmed