Stewart M , Donahue K , Wilke E , Shifley E .

P20 GM103436 NIGMS NIH HHS

	Figure 1. (A) LOC496300 is expressed in the endoderm of Xenopus laevis from NF stage 10 through 37. It becomes restricted to the midgut and hindgut during tailbud stages (~NF stg 30) and is expressed in the intestine and gall bladder of NF stage 42-43 gut tubes at differing levels (i.e. gut tube a and b). Embryos hybridized with the sense probe for LOC496300 show some non-specific staining in the head at tailbud stages, but overall no specific staining in the endoderm. NF stage 43 organ buds are marked as follows: lv, liver; p, pancreas; s, stomach; gb, gall bladder; i, intestine. (B) NF stage 10, 12, 28 and 35 embryos were sectioned to show the punctate expression pattern of LOC496300 throughout the early endoderm and in the dorsal and ventral tailbud endoderm. The numbered lines mark the approximate level of each section of the tailbud embryos and the dotted red line outlines the endoderm.
	XB5947952 (hypothetical LOC496300) gene expression in bissected X. laevis embryo, NF stage 10, dorsal right.
	XB5947952 (hypothetical LOC496300) gene expression in X. laevis embryo, NF stage 24, lateral view, dorsal up, anterior left.
	XB5947952 (hypothetical LOC496300) gene expression in isolated gut tubes of X. laevis embryo, NF stage 43, anterior left. NF stage 43 Key to developing foregut organs: lv, liver; p, pancreas; s, stomach; gb, gall bladder; i, intestine.

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