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Development 2013 Jun 01;14011:2409-21. doi: 10.1242/dev.093385.
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Tcf21 regulates the specification and maturation of proepicardial cells.

Tandon P , Miteva YV , Kuchenbrod LM , Cristea IM , Conlon FL .

The epicardium is a mesothelial cell layer essential for vertebrate heart development and pertinent for cardiac repair post-injury in the adult. The epicardium initially forms from a dynamic precursor structure, the proepicardial organ, from which cells migrate onto the heart surface. During the initial stage of epicardial development crucial epicardial-derived cell lineages are thought to be determined. Here, we define an essential requirement for transcription factor Tcf21 during early stages of epicardial development in Xenopus, and show that depletion of Tcf21 results in a disruption in proepicardial cell specification and failure to form a mature epithelial epicardium. Using a mass spectrometry-based approach we defined Tcf21 interactions and established its association with proteins that function as transcriptional co-repressors. Furthermore, using an in vivo systems-based approach, we identified a panel of previously unreported proepicardial precursor genes that are persistently expressed in the epicardial layer upon Tcf21 depletion, thereby confirming a primary role for Tcf21 in the correct determination of the proepicardial lineage. Collectively, these studies lead us to propose that Tcf21 functions as a transcriptional repressor to regulate proepicardial cell specification and the correct formation of a mature epithelial epicardium.

PubMed ID: 23637334
PMC ID: PMC3653561
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actc1 actl6a bmp2 ctbp2 huwe1 itga4 krt12.4 lhx9 nkx2-5 prkci psmd6 ptpn11 tbx18 tbx5 tcf12 tcf21 vim wt1
Morpholinos: tcf21 MO3 tcf21 MO4

GEO Series: GSE45786: Xenbase,  NCBI

Article Images: [+] show captions
References [+] :
Acharya, Efficient inducible Cre-mediated recombination in Tcf21 cell lineages in the heart and kidney. 2011, Pubmed