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Dev Cell 2008 Apr 01;144:616-23. doi: 10.1016/j.devcel.2008.01.009.
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Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline.

Christine KS , Conlon FL .

The CASTOR (CST) transcription factor was initially identified for its role in maintaining stem cell competence in the Drosophila dorsal midline. Here we report that Xenopus CST affects cardiogenesis. In CST-depleted embryos, cardiomyocytes at the ventral midline arrest and are maintained as cardiac progenitors, while cells in more dorsal regions of the heart undergo their normal program of differentiation. Cardia bifida results from failed midline differentiation, even though cardiac cell migration and initial cell fate specification occur normally. Our fate mapping studies reveal that this ventral midline population of cardiomyocytes ultimately gives rise to the outer curvature of the heart; however, CST-depleted midline cells overproliferate and remain a coherent population of nonintegrated cells positioned on the outer wall of the ventricle. These midline-specific requirements for CST suggest the regulation of cardiomyocyte differentiation is regionalized along a dorsal-ventral axis and that this patterning occurs prior to heart tube formation.

PubMed ID: 18410736
PMC ID: PMC2807748
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus
Genes referenced: a2m actl6a casz1 darmin gata4 gata5 gata6 h3-3a hcst myh6 nkx2-5 slc5a8 sox2 tbx20 tbx5 tpm1
Antibodies: Myh6 Ab1 Tpm1 Ab1
Morpholinos: casz1 MO1 casz1 MO2 casz1 MO3

Article Images: [+] show captions
References [+] :
Abu-Issa, Heart field: from mesoderm to heart tube. 2007, Pubmed