Figure 5 - Skin hypoplasia in Pycr1 morphants is accompanied by spontaneous apoptosis and is independent of anemia. (a) Scl expression at stage 25 marks site of primitive hematopoiesis in ventral ectoderm of control embryo. (b) Scl expression was abrogated in Pycr1 morphants suffering anemia (Supplementary Videos 1 and 2). (c) Parabiosis performed at stage 22 between a Pycr1-depleted (MO) tadpole (top) and control (bottom) did not rescue from apoptosis (inset, stage 33), stunted growth and skin defects despite shared blood circulation (Supplementary Video 3). (d) Histological staining. Epidermis of Pycr1 parabiotic morphant embryo remained disorganized relative to control. (e) Histological section through epidermis of parabiotic control tadpole at stage 45 showing normal skin architecture. (f) Acridine-orange background staining of control embryo (inset, bright-field image). (g) Acridine-orange labeling of dying cells (intense green) in Pycr1 morphant embryos injected only in the ventral (V) half (inset, bright-field image). Only the ventro-posterior half of the embryo, derived from the injected ventral blastomeres, is undergoing apoptosis. (h) TUNEL staining in stage 33 tadpole showing few apoptotic cells (inset, control at stage 25). (i) Pycr1 morphants, with a markedly increased number of dying cells at stage 33 relative to control (inset, stage 25). Image redisplayed with permission from Macmillan Publishers Ltd.
Image published in: Reversade B et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd.
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