Larger Image Fig. 2. Expression pattern of xOMP25 mRNA. (A) The temporal expression pattern of xOMP25 was determined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. XOMP25 mRNA exists maternally and expression is sustained until the tadpole stage. (B�F) The spatial expression pattern of xOMP25 mRNA is shown by whole-mount in situ hybridization. Signals are observed in the animal pole at the 8 cell stage (B), in the anterior neural fold at the stage 18 (C,D), and in eye, otic vesicle, branchial arch and neural tube in stages 28 and 35 (E,F). xOMP25 expression in the otic vesicle and neural tube was also seen in transverse section (G). Anterior upward view (C). Image published in: Inui M and Asashima M (2004) Copyright © 2004. Image reproduced with permission of the Publisher, John Wiley & Sons.
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