Figure 4. Immunohistochemistry of XAK-C and XAK-B. Sections of skin tissues were prepared from tadpoles at stage 56 (St. 56, A), 58 (B,C), and 61 (D), and adults (G) and subjected to immunohistochemistry for XAK-C (A,B,D,G) and XAK-B (C,E,H). Red and green color represent stains of XAK-C and XAK-B, respectively. F and I are the merged images of D and E, and G and H, respectively. There was no XAK-C signal at stage 56. Basal cells at stage 58 were XAK-C positive, but no cells were positive for XAK-B. Basal cells of the skin at stage 61 and of adults were stained with antiAK-C antisera. XAK-B signals were detected in differentiated cells seen on both sections. Immunohistochemistry experiments were performed four times, and similar staining patterns were obtained reproducibly. Scale bar = 20 μm in I (applies to A).
Image published in: Watanabe Y et al. (2002)
Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| krt55.L | adult-specific keratin-b, krt5.L, xak-b | X. laevis | Throughout NF stage 56 to NF stage 58 | |
| krt55.L | adult-specific keratin-b, krt5.L, xak-b | X. laevis | Sometime during NF stage 61 to NF stage 66 | skin epidermis |
| krt12.6.S | adult-specific keratin, xak-c | X. laevis | Throughout NF stage 56 | |
| krt12.6.S | adult-specific keratin, xak-c | X. laevis | Throughout NF stage 58 to NF stage 66 | skin epidermis basal lamina |
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