Fig. 1. Localization of TRPN1 in lateral-line hair cells of stage-45 Xenopus embryos. (A and B) Dissecting microscope dorsal view of embryos that had been stained with FM1-43 to label lateral-line hair cells. In A, red arrows point to lateral-line hair cells around the eye; in B, white arrows highlight the absence of FM1-43 staining around the eyes of neomycin-treated embryos. (C and D) Shown are A and B merged with their respective transmitted light images to better show the localization of FM1-43 staining. (E) Staining with the anti-TRPN1 antibody. Dorsal view of an embryo that had been bleached to distinguish alkaline phosphatase staining from pigment. Red arrows point to staining of the lateral-line stitches around the eye. Blue arrows point to staining of the nostrils. (F) Anti-TRPN1 staining of unbleached, neomycin-treated embryo. Nostrils are positive for TRPN1, but staining around the eyes is absent, showing that the neomycin effect is specific to lateral-line hair cells. (G) Close-up of the lateral-line staining surrounding the eye shown in E. Red arrows point to signal in lateral-line stitches. (H) Dorsal view of bleached embryo. Preadsorption of the TRPN1 antibody with the epitope peptide prevented TRPN1 staining of both the lateral line and the nostril (white arrows).
Image published in: Shin JB et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| XB5813854.L | nompc, trpn1 | X. laevis | Throughout NF stage 45 | nostril lateral line system supraorbital lateral line lateral line olfactory system olfactory sensory neuron olfactory organ |
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