Larger Image Fig. 5. In situ localization of ID14 mRNA to the intestinal epithelium. Cross-sections of the intestine were hybridized with the antisense (AâD) or sense (E) RNA probes. (A) No specific signals were detected in the larval epithelium (le) at stage 54. (B) No signals were seen in dying larval cells (le) or proliferating, undifferentiated adult epithelial cells (ae) at stages 61 and 62. Identification of the area of adult cell proliferation demarcated by dark line is based on previous studies (Ishizuya-Oka and Ueda, 1996). (C) Signals became detectable in the adult epithelium (ae) at stage 63, and intensified by stage 66 (D). (E) Background signal was weakly seen for the intestine at stage 66 hybridized with the sense probe. ct, connective tissue; m, muscles. Bars, 20 μm. Image published in: Buchholz DR et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.
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