Figure 8. Knock down of Dvl3 in presomitic mesodermal tissues disrupts normal expression of both paraxis and Pax-1 genes. A,B: Single-stage 29/30 tailbud stage embryo. A: Control uninjected side displays normal paraxis expression in the posterior presomitic mesoderm and within individual somites. a': A magnified image of posterior half of the (A) side of the embryo. B: Morpholino-injected side displays a loss of paraxis expression within individual somites as well as diminished expression in the unsegmented mesoderm. b': A magnified image of the posterior half of the (B) side of the embryo. C: Normal expression of Pax-1 in a wild type stage-37 embryo in the branchial arches and perinotochordal region. D: Dvl3 morphants lack any appreciable Pax-1 staining surrounding the notochord (targeted by injection) while the brachial arches (not targeted by injection) retain Pax-1 expression. Red arrows point to paraxis expression within individual somites. Red bracket highlights defects in both expression and patterning of paraxis transcript expression.
Image published in: Gray RS et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tcf15.L | paraxis | X. laevis | Throughout NF stage 29 and 30 | somite presomitic mesoderm |
| pax1.S | LOC108716602, pax-1 | X. laevis | Throughout NF stage 37 and 38 | branchial arch sclerotome perichordal tissue mandibular arch |
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