Larger Image Figure S4: Loss of dyrk1a increases cleaved caspase 3 and phospho-histone H3 staining. (A-B) Representative images of cleaved caspase 3 (yellow) staining of X. tropicalis neurula stage embryos injected only on the right side with dyrk1a morpholino (MO) (A) or dyrk1a CRISPR/Cas9 reagents (B). (C-D) Representative images of phospho-histone H3 (magenta) staining of X. tropicalis neurula stage embryos treated with DMSO (C) or 10 μM Harmine (D) beginning at blastula stages. Outlines of the embryos are shown in white dashed lines (C-D). All images are dorsal view with the anterior oriented to the top, and maximum intensity projections of optical sections. All conditions have a sample size greater than 20. Image published in: Willsey HR et al. (2020) Copyright © 2020. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xtr WT + dyrk1a MO + NF2-18 (immunohistochemistry) Fig S4 A Expression Phenotype increased amount casp3.2 expression in neural plate Xtr WT + dyrk1a CRISPR + NF2-18 (immunohistochemistry) Fig S4 B Expression Phenotype increased amount casp3.2 expression in neural plate Xtr Wt + Harmine + NF2-10.5 (cell death detection assay) Fig S4 D Anatomical Phenotype abnormal cell division Permanent Image Page Printer Friendly View XB-IMG-184296