Figure 3. Pattern of localization of Trpc1 in gastrula stage embryos. (A) Single optical view of a sagittal section taken though a gastrula stage embryo, showing the localization of Trpc1 in the ectoderm (ect) and mesoderm (mes). There is a lower level of Trpc1 expression in the endoderm (end). The arrow indicates the position of the blastopore lip. The regions bounded by the dashed white rectangles are shown at higher magnification in panels (B–D). These images show that at the cellular level, Trpc1 is localized mainly in the plasma membrane. Trpc1 localization, shown in green, was revealed with the rabbit anti-Trpc1 polyclonal antibody and an Alexa-555-conjugated anti-rabbit secondary antibody. The nuclei (in blue) were labelled with ToPro3. Scale bars are 300 µm in panel (A), and 40 µm in panels (B–D).
Image published in: Néant I et al. (2019)
© The Author(s) 2019. Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| trpc1.L | xtrpc1 | X. laevis | Sometime during NF stage 10 to NF stage 10.5 | ectoderm mesoderm plasma membrane |
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