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Larger Image Figure 3. Xenopus GDA is expressed in the developing kidney.A. RT-PCR showing gdaexpression in the different developmental stages: 2 cell, blastula, neurula, 3 dpf, 6 dpf, and adult. ODC1 was used as an internal RT-PCR reference. B. Whole-mount in situ hybridization using an antisense probe specific to gda in different developmental stages. Scale bar: 0.5 mmC. Whole-mount in situ hybridization using an antisense probe specific to GDA or the kidney marker xSGLT-1K in 4.5 dpf embryos. Both gda and xSGLT-1K can be seen in a similar embryonic location. Scale bar: 0.5 mm and 0.25 mm for magnifications. Image published in: Slater PG et al. (2019) Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.
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