Fig. 1. Spatial-temporal expression profiles of SRSF1 during Xenopus embryogenesis. (A) Spatial expression of SRSF1 was determined by whole-mount in situ hybridization of Xenopus embryos from stage 0–32. SRSF1 is mainly localized in the animal hemisphere in an early embryo (∼stage 6) and sequentially widens to the vegetal hemisphere through gastrulation (∼stage 12). At the late neurula stage, SRSF1 is expressed in the neural plate- (∼stage 18) and then is strongly expressed in the central nervous system (CNS), including the front head, eye, and branchial arches (∼stage 22 and stage 32). (B) Reverse transcriptase-PCR (RT-PCR) results to show temporal expression patterns of SRSF1. SRSF1 is continuously expressed from stage 0 embryos to stage 40 tadpoles and expression levels reduced from stage 0–8 were restored at gastrula stage (stage 17). This restored expression again decreased after the neurula stage but also increased and peaked at stage 35. Ornithine decarboxylase (ODC), was used as a loading control.
Image published in: Lee SH et al. (2016)
Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| srsf1.L | asf, sf2, sf2p33, sfrs1, srp30a | X. laevis | Throughout unfertilized egg stage | animal pole |
| srsf1.L | asf, sf2, sf2p33, sfrs1, srp30a | X. laevis | Sometime during NF stage 6 (32-cell) to NF stage 12 | animal hemisphere |
| srsf1.L | asf, sf2, sf2p33, sfrs1, srp30a | X. laevis | Throughout NF stage 18 | neural plate cranial neural crest |
| srsf1.L | asf, sf2, sf2p33, sfrs1, srp30a | X. laevis | Throughout NF stage 22 | neural tube |
| srsf1.L | asf, sf2, sf2p33, sfrs1, srp30a | X. laevis | Throughout NF stage 32 | central nervous system brain spinal cord eye pharyngeal arch |
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