Fig. 2 Expression of Na,K-ATPase b subunit genes during Xenopus embryogenesis. Whole-mount in situ hybridizations were per- formed on Xenopus embryos with antisense probes for b1, b2, and b3 subunits. Sections of stage 29 (S), stage 30 (T) and stage 37 (R) embryos stained in whole-mount were cut at 30â40 mm. Dorsal (A, C, D) and lateral (E-N) views are shown with anterior to the left. Frontal views (B, O-Q) and sections (R-T) are oriented with dorsal to the top. A Stage 19 embryo with punctate b1 expression in the epidermis. B, C b2 expression was confined to the non-neural ectoderm at stage 15 (B) and throughout the epidermis at stage 19 (C). D Stage 19 embryo showing b3 expression in the developing brain and neural tube. E-G b1 transcripts appeared at stage 26 (E) initially in the otic vesicles, pronephric anlage, somites, and proctodeum. Later, at stage 29 (F) staining was noticeable in the trigeminal (V) nerves. By stage 37 (G), b1 transcripts were also evident in the olfactory bulbs, facial (VII) and glossopharyngeal (IX) nerves. H-J Extensive epidermal expression of b2 transcripts was observed in stage 25 (H), stage 29 (I), and stage 38 (J) embryos. Arrowhead indicates high levels of b2 in the gills. Note the increasing b2 expression in the brain and eyes. K-M b3 gene expression was found at stage 26 (K) in the brain, eyes, otic vesicles, somites, and cranial neural crest (arrowheads). By stage 30 (L), strong stain ing was evident at the forebrain-midbrain junction (arrowhead), in the region of the hepatic primordium, the proctodeum, and olfac- tory bulbs. b3 transcripts were seen at stage 38 laterally in the migrating abdominal muscle anlagen (arrowheads). N High magni fication of a stage 37 embryo illustrating b1 expression in the pronephric kidney. O-Q Frontal views showing b1 (O), b2 (P), and b3 (Q) expression in the developing head. R-T Transverse sections through the midtrunk of stage 37 (R) and at the level of the pro nephros of stage 29 (S) and stage 30 (T) embryos. b1 transcripts (R) were present in the pronephric duct, b2 expression was confined to the epidermis, and b3 was observed in spinal nerves and the hepatic primordium. Abbreviations: br, brain; e, eye; ed, ectoderm; ep, epidermis; hp, hepatic primordium; m, mouth, np, neural plate; nt, neural tube; ob, olfactory bulb; ov, otic vesicle; p, proctodeum; pd, pronephric duct; pt, pronephric tubules; pn, pronephros; sc, spinal cord; sm, somites; sn, spinal neurons.
Image published in: Eid SR and Brändli AW (2001)
Copyright © 2001. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| atp1b1.L | atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase | X. laevis | Sometime during NF stage 19 to NF stage 25 | intermediate mesoderm pronephric mesenchyme otic vesicle proctodeum somite |
| atp1b2.S | X. laevis | Sometime during NF stage 24 to NF stage 39 | epidermis non-neural ectoderm | |
| atp1b3.L | atpb-3, beta-3, β3 ATPase subunit | X. laevis | Sometime during NF stage 24 to NF stage 39 | optic vesicle otic vesicle olfactory placode olfactory bulb neural tube heart primordium trigeminal nerve trigeminal placode pineal gland liver primordium proctodeum spinal cord hypaxial muscle spinal neuron |
| atp1b1.L | atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase | X. laevis | Sometime during NF stage 25 to NF stage 39 | pronephric kidney pronephric nephron pronephric tubule distal tubule proximal tubule late proximal tubule early proximal tubule rectal diverticulum pronephric duct otic vesicle cranial nerve trigeminal nerve facial nerve glossopharyngeal nerve olfactory bulb |
| atp1b1.L | atp1b1a, Beta1-Na/K-ATPase, Na+-K+-ATPase, β1-NaK-ATPase | X. laevis | Throughout NF stage 26 | subpallium lateral ganglionic eminence medial ganglionic eminence prethalamus |
| atp1b2.S | X. laevis | Throughout NF stage 39 | gill primordium brain eye pretectum |
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