FIG. 4. Whole mount in situ hybridization with digoxigenin-labeled XDCCa riboprobes. Stage 37/38 Xenopus embryos were studied by whole mount in situ hybridization using a 630-bp anti-sense (A) or sense XDCCa (B) riboprobe. The probes were digoxigenin-labeled and alkaline phosphatase-conjugated, anti-digoxigenin antibodies were used to detect hybridization signals. Specific hybridization to developing forebrain, midbrain, and hindbrain regions was seen with the anti-sense XDCCa probe (A, arrowheads). Nonspecific staining of some areas was seen with both the sense and anti-sense XDCCa probes.
Image published in: Pierceall WE et al. (1994)
Copyright © 1994. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| dcc.S | crc18, crcr1, dcc-a, dcc-b, igdcc1, xdcc | X. laevis | Throughout NF stage 37 and 38 | brain forebrain midbrain hindbrain nervous system central nervous system midbrain-hindbrain boundary |
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