Fig. 2. Tissue-specific expression of nadk during sea urchin and frog development. (A) Images show in situ hybridization reactions recognizing S. purpuratus nadk expression during development. Open red arrowheads indicate the ciliary band. The closed red arrowhead points to cells in the aboral ectoderm of the pluteus that constitute the vertex structure. The yellow arrowhead points to the gut. hpf, hours postfertilization. (B) RT-qPCR expression profiling of Spnadk-1 and Spnadk-2 during S. purpuratus embryonic development. (C) Images of in situ hybridization reactions recognizing X. tropicalis nadk expression during various stages of development (16). Closed black arrowheads indicate primitive myeloid cells. Open black arrowheads point to the cement gland. (D) Expression of nadk following injection of cebpα morpholino, a loss-of-function technique that removes PMCs from the embryo (16). The black arrowhead indicates a PMC. (E) Schematic diagram outlining the experimental procedure of ectopically inducing PMC development in X. tropicalis animal caps using the injection of cebpα mRNA (16). (F) Expression of nadk in cebpα or egfp mRNA-injected animal caps as detected by RT-qPCR (n = 3). Error bars indicate SD of three independent experiments.
Image published in: Love NR et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| nadk | nadk-a, nadk-b, NAD kinase | X. tropicalis | Throughout NF stage 10.5 | marginal zone whole organism |
| nadk | nadk-a, nadk-b, NAD kinase | X. tropicalis | Throughout NF stage 18 | anterior ventral blood island |
| nadk | nadk-a, nadk-b, NAD kinase | X. tropicalis | Throughout NF stage 23 | ventral blood island anterior ventral blood island myeloid cell |
| nadk | nadk-a, nadk-b, NAD kinase | X. tropicalis | Throughout NF stage 26 | myeloid cell |
| nadk | nadk-a, nadk-b, NAD kinase | X. tropicalis | Sometime during NF stage 6 (32-cell) to NF stage 8 | whole organism |
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