FIGURE 1. Expression pattern of scp3 in Xenopus. (A-B) At 8-cell stage, total mRNA from animal and vegetal blastomeres was extracted respectively and analyzed by RNA-seq (A) or qPCR (B). The numbers in (A) indicate the RPKM (reads per kilobase per million mapped reads) of sequencing in two independent experiments (54). Vegetal enriched transcripts bicc was used as a control. Asterisk indicates significantly different (A, FDR<0.05; B, P-value<0.05) between animal and vegetal samples. (C) At indicated stages, RNA from animal and vegetal cells was extracted respectively and the relative mRNA abundance of scp3 was analyzed by qPCR. (D) qPCR shows the temporal expression pattern of scp3. (E) Expression profile of scp3 verified by in situ hybridization. The embryos at 1-cell to stage 10.5 were shown in lateral view. Stage 17 embryo was shown in dorsal view with the anterior end towards the left side. Stage 28 embryo was shown in lateral view with its head towards the left. The arrow indicates the dorsal lip.
Image published in: Sun G et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| ctdspl.S | scp3, xnif | X. laevis | Throughout NF stage 1 | vegetal vegetal pole |
| ctdspl.S | scp3, xnif | X. laevis | Throughout NF stage 4 (8-cell) | vegetal blastomere vegetal |
| ctdspl.S | scp3, xnif | X. laevis | Throughout NF stage 10.5 | vegetal yolk mass endoderm |
| ctdspl.S | scp3, xnif | X. laevis | Throughout NF stage 17 | paraxial mesoderm presomitic mesoderm |
| ctdspl.S | scp3, xnif | X. laevis | Throughout NF stage 28 | muscle epaxial muscle somite |
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