Fig. 3. zcchc24 expression during neurula stages. Whole mount in situ hybridization using DIG labelled zcchc24 (A-Bââ, E) or fluorescein labelled nkx2.5 (D) and double whole mount in situ hybridization with DIG labelled zcchc24 and fluorescein labelled nkx2.5 (C) were performed on embryos at neurula stages. (A) At late gastrula/early neurula (st 13), zcchc24 is expressed as two stripes in the dorsal side of the embryo along the antero-posterior axis but is excluded from the notochord. (B-Bââ, E) During neurula stages, zcchc24 is expressed in anterior and somitogenic mesoderm, decreasing the expression from the posterior to the anterior region. Comparison between the expression of zcchc24 and nkx2.5 (B,C,D) shows that the most anterior expression of zcchc24 is adjacent to the cardiac mesoderm. (A,B, C-E) Embryos in a dorsal view. (Bâ, Bââ) Transversal sections of st 15 embryos with the dorsal region displayed to the top. Red arrows indicate the heart precursor region. A, anterior; P, posterior; n, notochord; sm, somitogenic mesoderm.
Image published in: Vitorino M et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| zcchc24.S | LOC108703996 | X. laevis | Sometime during NF stage 13 to NF stage 18 | paraxial mesoderm presomitic mesoderm cardiac mesoderm |
| nkx2-5.L | AR2, csx, nkx-2.5, Nkx-2.5, nkx2.5, nkx2-5-a, nkx2-5-b, tinman, XNkx-2.5, XNkx2-5 | X. laevis | Throughout NF stage 18 | cardiac mesoderm |
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