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Front Cell Dev Biol 2024 Jan 01;12:1365705. doi: 10.3389/fcell.2024.1365705.
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A maternal dorsoventral prepattern revealed by an asymmetric distribution of ventralizing molecules before fertilization in Xenopus laevis.

Castro Colabianchi AM , González Pérez NG , Franchini LF , López SL .

The establishment of the embryonic dorsoventral axis in Xenopus occurs when the radial symmetry around the egg's animal-vegetal axis is broken to give rise to the typical symmetry of Bilaterians. We have previously shown that the Notch1 protein is ventrally enriched during early embryogenesis in Xenopus laevis and zebrafish and exerts ventralizing activity through β-Catenin destabilization and the positive regulation of ventral center genes in X. laevis. These findings led us to further investigate when these asymmetries arise. In this work, we show that the asymmetrical distribution of Notch1 protein and mRNA precedes cortical rotation and even fertilization in X. laevis. Moreover, we found that in unfertilized eggs transcripts encoded by the ventralizing gene bmp4 are also asymmetrically distributed in the animal hemisphere and notch1 transcripts accumulate consistently on the same side of the eccentric maturation point. Strikingly, a Notch1 asymmetry orthogonal to the animal-vegetal axis appears during X. laevis oogenesis. Thus, we show for the first time a maternal bias in the distribution of molecules that are later involved in ventral patterning during embryonic axialization, strongly supporting the hypothesis of a dorsoventral prepattern or intrinsic bilaterality of Xenopus eggs before fertilization.

PubMed ID: 38572484
PMC ID: PMC10987785
Article link: Front Cell Dev Biol

Species referenced: Xenopus tropicalis Xenopus laevis
Genes referenced: bmp4 dll1 elob gdf1 hes4 hwa mpi notch1 pou5f3 tcea1 tubb2b wnt11b wnt8b znf330
GO keywords: embryo development
Antibodies: Digoxigenin AB1 Notch1 AB1

Article Images: [+] show captions
References [+] :
Acosta, Notch destabilises maternal beta-catenin and restricts dorsal-anterior development in Xenopus. 2011, Pubmed, Xenbase