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XB-ART-59250
Development 2022 Sep 01;14917:. doi: 10.1242/dev.200552.
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Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants.

Houston DW , Elliott KL , Coppenrath K , Wlizla M , Horb ME .


Abstract
Asymmetric signalling centres in the early embryo are essential for axis formation in vertebrates. These regions (e.g. amphibian dorsal morula, mammalian anterior visceral endoderm) require stabilised nuclear β-catenin, but the role of localised Wnt ligand signalling activity in their establishment remains unclear. In Xenopus, dorsal β-catenin is initiated by vegetal microtubule-mediated symmetry breaking in the fertilised egg, known as 'cortical rotation'. Localised wnt11b mRNA and ligand-independent activators of β-catenin have been implicated in dorsal β-catenin activation, but the extent to which each contributes to axis formation in this paradigm remains unclear. Here, we describe a CRISPR-mediated maternal-effect mutation in Xenopus laevis wnt11b.L. We find that wnt11b is maternally required for robust dorsal axis formation and for timely gastrulation, and zygotically for left-right asymmetry. Importantly, we show that vegetal microtubule assembly and cortical rotation are reduced in wnt11b mutant eggs. In addition, we show that activated Wnt coreceptor Lrp6 and Dishevelled lack behaviour consistent with roles in early β-catenin stabilisation, and that neither is regulated by Wnt11b. This work thus implicates Wnt11b in the distribution of putative dorsal determinants rather than in comprising the determinants themselves. This article has an associated 'The people behind the papers' interview.

PubMed ID: 35946588
PMC ID: PMC9515810
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dvl2 krt12.4 lrp6 mag mapk1 mapre3 myod1 nanos1 nodal3.1 nodal3.4 pitx2 psmd6 rab11a rab5a sia1 sox17a szl vegt wnt11 wnt11b wnt8a
GO keywords: Wnt signaling pathway [+]
Morpholinos: wnt11b MO2

GEO Series: GSE195806: NCBI

Article Images: [+] show captions
References [+] :
Applegate, plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics. 2011, Pubmed